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p re ss alexa488 conjugated anti digoxigenin  (Jackson Immuno)


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    Jackson Immuno p re ss alexa488 conjugated anti digoxigenin
    P Re Ss Alexa488 Conjugated Anti Digoxigenin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+digoxigenin/Alexa+Fluor+488+IgG+Fraction+Monoclonal+Mouse+Anti-Digoxin/pm41559265-302-6-11
    Average 93 stars, based on 51 article reviews
    p re ss alexa488 conjugated anti digoxigenin - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunodetection:

    Article Title: Origin and Evolution of Diploid and Allopolyploid Camelina Genomes Were Accompanied by Chromosome Shattering
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by Mandáková and Lysak (2016b) as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Article Title: Evolution of Tandem Repeats Is Mirroring Post-polyploid Cladogenesis in Heliophila (Brassicaceae)
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red, and digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Avidin-Biotin Assay:

    Article Title: Origin and Evolution of Diploid and Allopolyploid Camelina Genomes Were Accompanied by Chromosome Shattering
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by Mandáková and Lysak (2016b) as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Article Title: Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae.
    Article Snippet: Biotin-dUTP was detected by Avidin Texas RedTM (cat. no. A-2006-5; Vector Laboratories, https:// vectorlabs.com) and amplified by goat anti-avidin biotin (BA-03005; Vector Laboratories) and Avidin Texas RedTM; digoxigenin-dUTP was detected by mouse anti-digoxigenin (200-002-156; Jackson ImmunoResearch, https://www.jacksonimmuno.com) and goat anti-mouse Alexa Fluor 488 (A11001; Invitrogen, now ThermoFisher Scientific, https://www.thermofisher.com).

    Article Title: Chromosomal Phylogeny and Karyotype Evolution in x=7 Crucifer Species (Brassicaceae)
    Article Snippet: .. Fluorescent detection was as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse Alexa Fluor 488 (Molecular Probes), and Cy3-dUTP labeled probes were observed directly. .. Chromosomes were counterstained with 4′,6-diamidino-2-phenylindole (2 μg/mL) in Vectashield (Vector Laboratories).

    Article Title: Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae.
    Article Snippet: Wenbo Shan, Michaela Kubov a, Terezie Mand akov a and Martin A. Lysak Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, Brno 625 00, Czech Republic, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, Brno 625 00, Czech Republic, and Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, Brno 625 00, Czech Republic

    Article Title: Evolution of Tandem Repeats Is Mirroring Post-polyploid Cladogenesis in Heliophila (Brassicaceae)
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red, and digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Amplification:

    Article Title: Origin and Evolution of Diploid and Allopolyploid Camelina Genomes Were Accompanied by Chromosome Shattering
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by Mandáková and Lysak (2016b) as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Article Title: Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae.
    Article Snippet: Biotin-dUTP was detected by Avidin Texas RedTM (cat. no. A-2006-5; Vector Laboratories, https:// vectorlabs.com) and amplified by goat anti-avidin biotin (BA-03005; Vector Laboratories) and Avidin Texas RedTM; digoxigenin-dUTP was detected by mouse anti-digoxigenin (200-002-156; Jackson ImmunoResearch, https://www.jacksonimmuno.com) and goat anti-mouse Alexa Fluor 488 (A11001; Invitrogen, now ThermoFisher Scientific, https://www.thermofisher.com).

    Article Title: Chromosomal Phylogeny and Karyotype Evolution in x=7 Crucifer Species (Brassicaceae)
    Article Snippet: .. Fluorescent detection was as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse Alexa Fluor 488 (Molecular Probes), and Cy3-dUTP labeled probes were observed directly. .. Chromosomes were counterstained with 4′,6-diamidino-2-phenylindole (2 μg/mL) in Vectashield (Vector Laboratories).

    Article Title: Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae.
    Article Snippet: Wenbo Shan, Michaela Kubov a, Terezie Mand akov a and Martin A. Lysak Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, Brno 625 00, Czech Republic, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, Brno 625 00, Czech Republic, and Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, Brno 625 00, Czech Republic

    Article Title: Evolution of Tandem Repeats Is Mirroring Post-polyploid Cladogenesis in Heliophila (Brassicaceae)
    Article Snippet: .. The immunodetection of hapten-labeled probes was performed as described by as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red, and digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse–Alexa Fluor 488 (Invitrogen). ..

    Labeling:

    Article Title: Chromosomal Phylogeny and Karyotype Evolution in x=7 Crucifer Species (Brassicaceae)
    Article Snippet: .. Fluorescent detection was as follows: biotin-dUTP was detected by avidin–Texas Red (Vector Laboratories) and amplified by goat anti-avidin–biotin (Vector Laboratories) and avidin–Texas Red; digoxigenin-dUTP was detected by mouse anti-digoxigenin (Jackson ImmunoResearch) and goat anti-mouse Alexa Fluor 488 (Molecular Probes), and Cy3-dUTP labeled probes were observed directly. .. Chromosomes were counterstained with 4′,6-diamidino-2-phenylindole (2 μg/mL) in Vectashield (Vector Laboratories).

    In Situ Hybridization:

    Article Title: Depletion of CD4+ T cells abrogates post-peak decline of viremia in SIV-infected rhesus macaques
    Article Snippet: After washing in RWS buffer, 2× SSC, and 0.1× SSC at 37°C for 15 minutes each, sections were transferred to 1× Tris-buffered saline (TBS; Boston BioProducts) containing 0.05% Tween-20 (TBS-Tw). .. Tissues were blocked in TBS containing either 2% sheep serum (double ISH/IHC) or 2% donkey serum (fluorescence ISH) for 1 hour at RT and incubated with either sheep Fab anti-digoxigenin (Roche Applied Science) at 1:500 in TBS containing 2% sheep serum (double ISH/IHC) or mouse anti-digoxigenin (Jackson ImmunoResearch) at 1:2,000 in TBS containing 2% donkey serum (for fluorescence ISH) with rabbit monoclonal anti-CD3 (clone SP7; Labvision) for 1 hour at RT or overnight at 4°C. .. For double SIV ISH/IHC, tissues were washed in TBS-Tw, incubated with Polink-1 Rabbit HRP 1-step polymer system (Golden Bridge International Labs) for 30 minutes, developed with ImmPact NovaRED peroxidase substrate (Vector Laboratories) for 2–10 minutes, washed in TBS-Tw, incubated with the alkaline phosphatase substrate NBT/BCIP containing levamisole (Roche Applied Science) for 2–6 hours, placed into double-distilled H2O, covered in Clear-Mount (Electron Microscopy Systems), dried, cleared in xylenes, and coverslipped with Permount (Fisher).

    Fluorescence:

    Article Title: Depletion of CD4+ T cells abrogates post-peak decline of viremia in SIV-infected rhesus macaques
    Article Snippet: After washing in RWS buffer, 2× SSC, and 0.1× SSC at 37°C for 15 minutes each, sections were transferred to 1× Tris-buffered saline (TBS; Boston BioProducts) containing 0.05% Tween-20 (TBS-Tw). .. Tissues were blocked in TBS containing either 2% sheep serum (double ISH/IHC) or 2% donkey serum (fluorescence ISH) for 1 hour at RT and incubated with either sheep Fab anti-digoxigenin (Roche Applied Science) at 1:500 in TBS containing 2% sheep serum (double ISH/IHC) or mouse anti-digoxigenin (Jackson ImmunoResearch) at 1:2,000 in TBS containing 2% donkey serum (for fluorescence ISH) with rabbit monoclonal anti-CD3 (clone SP7; Labvision) for 1 hour at RT or overnight at 4°C. .. For double SIV ISH/IHC, tissues were washed in TBS-Tw, incubated with Polink-1 Rabbit HRP 1-step polymer system (Golden Bridge International Labs) for 30 minutes, developed with ImmPact NovaRED peroxidase substrate (Vector Laboratories) for 2–10 minutes, washed in TBS-Tw, incubated with the alkaline phosphatase substrate NBT/BCIP containing levamisole (Roche Applied Science) for 2–6 hours, placed into double-distilled H2O, covered in Clear-Mount (Electron Microscopy Systems), dried, cleared in xylenes, and coverslipped with Permount (Fisher).

    Incubation:

    Article Title: Depletion of CD4+ T cells abrogates post-peak decline of viremia in SIV-infected rhesus macaques
    Article Snippet: After washing in RWS buffer, 2× SSC, and 0.1× SSC at 37°C for 15 minutes each, sections were transferred to 1× Tris-buffered saline (TBS; Boston BioProducts) containing 0.05% Tween-20 (TBS-Tw). .. Tissues were blocked in TBS containing either 2% sheep serum (double ISH/IHC) or 2% donkey serum (fluorescence ISH) for 1 hour at RT and incubated with either sheep Fab anti-digoxigenin (Roche Applied Science) at 1:500 in TBS containing 2% sheep serum (double ISH/IHC) or mouse anti-digoxigenin (Jackson ImmunoResearch) at 1:2,000 in TBS containing 2% donkey serum (for fluorescence ISH) with rabbit monoclonal anti-CD3 (clone SP7; Labvision) for 1 hour at RT or overnight at 4°C. .. For double SIV ISH/IHC, tissues were washed in TBS-Tw, incubated with Polink-1 Rabbit HRP 1-step polymer system (Golden Bridge International Labs) for 30 minutes, developed with ImmPact NovaRED peroxidase substrate (Vector Laboratories) for 2–10 minutes, washed in TBS-Tw, incubated with the alkaline phosphatase substrate NBT/BCIP containing levamisole (Roche Applied Science) for 2–6 hours, placed into double-distilled H2O, covered in Clear-Mount (Electron Microscopy Systems), dried, cleared in xylenes, and coverslipped with Permount (Fisher).



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